MegaMix HRM - High Resolution Melting PCR Mastermix
MegaMix HRM contains all the components needed to perform HRM-PCR swiftly and reliably. The 2X mix contains chemically modified Hot Start Taq DNA polymerase and microGREEN intercalating dye in enhancing buffer optimised for HRM analysis. microGREEN is a third generation, saturating, intercalating fluorescent dye that binds to double stranded DNA without inhibiting PCR; making MegaMix HRM the perfect choice for HRM.
The Hot Start Taq polymerase is chemically inactivated until heating to 95°C, providing excellent sensitivity and specificity; eliminating theformation of non-specific amplification and primer-dimers.
This mastermix performs excellently when amplifying GC rich templates, providing reproducible and reliable results with little or no optimization.
