The Flash Protein Ladder contains a three color, 10 protein ladder that resolve into sharp, tight bands in the range of 6-270 kDa. The Flash Protein Ladder allows you to monitor molecular weight separation during electrophoresis, estimate molecular weights of proteins of interest, and evaluate western transfer efficiency. Total volume = 500uL
The Flash pre-stained Protein Ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis and verifying Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for estimating protein mass. The ladder is supplied in gel loading buffer for immediate use.
Use 3uL to 5uL per well for in gel visualization, or 2.5uL per well for western blotting.
Storage Buffer: 62.5 mM Tris-H3PO4 (pH 7.5 at 25 °C), 1 mM EDTA, 2% SDS, 10 mM DTT, 1 mM NaN3, 33% glycerol.
Stable for up to 3 months at 4°C.
Stable for up to 2 weeks at 25°C.
For long-term storage, store at -20°C.
