microLYSIS-Plus® will help you get DNA from even the most difficult sources. It can lyse the toughest bacterial cells and spores as well as yeasts, fungi, moulds, some plant and some animal tissues directly. Mix cells with this more complex lysis solution and thermocycle to release DNA. Unlike DNA released with bead-beating systems, DNA released using microLYSIS-Plus is intact.
microLYSIS-Plus® has been successfully used to get DNA from:
Mammalian: Saliva, Fibroblasts, Hair, African fruit bat wing, Sperm, HeLa carcinoma cells, Keratinocytes, Tissues, Paraffin embedded tissues, Mouse tail & Mouse ear
Plants: Peanut, Rice, Grass seed, Lettuce, Pepper, Paw-paw, Arabidopsis, Soya, Maize, Cabbage, Oilseed Rape, Wheat & Oats
Fungal: Phakospora euvitis, Sclerotinia sclerotiorum, Microdochium nivale, Fusarium spp, Pochonia chlamydosporia, Pseudogymnoascus destructans, Rynchosporium spp & Pythium aphanidermatum (fungus like)
Yeast: Saccharomyces cerevisiae, Schizosaccharomyces pombe & Candida spp
Gram+ bacteria: Actinomyces spp, Bacillus spp, Brevibacterium linens, Clostridium botulinum, Corynebacterium casei, Enterococcus spp, Lactobacillus spp, Leuconostoc spp, Macrococcus caseolyticus, Micrococceae strains, Microcystis spp, Pasteuria spp, Propionibacterium freudenrechii, Staphylococcus spp, Streptococcus spp & Streptomyces coelicolor
Gram- bacteria: Coliform Bacilli, Escherichia coli, Hemophilus influenzae, Leptospira pomona, Nitrosomonas, Pseudomonas, Vibrio parahaemolyticus, Halobacteria spp, Sulfolobus, Cyanobacteria spp, Campylobacter jejuni, Neissera meningitides & Veillonella
Other: Drosophila, Barnacle Larvae, Bird feathers – Ostrich, Tardigrades, Fleas, Plankton, Nematodes – Schistosomes, Encapsulated nematode larvae, Baculovirus, Slime moulds, Various species of meiofauna & Protozoa – Crytosporidium
